| RRC ID |
73657
|
| 著者 |
Karakeçili A, Topuz B, Ersoy FŞ, Şahin T, Günyakti A, Demirtaş TT.
|
| タイトル |
UiO-66 metal-organic framework as a double actor in chitosan scaffolds: Antibiotic carrier and osteogenesis promoter.
|
| ジャーナル |
Biomater Adv
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| Abstract |
Metal-organic frameworks (MOFs) have recently emerged as a useful class of nanostructures with well-suited characteristics for drug delivery applications, due to the high surface area and pore size for efficient loading. Despite their use as a nano-carrier for controlled delivery of various types of drugs, the inherent osteo-conductive properties have stolen a great attention as a growing area of investigation. Here, we evaluated the double function of UiO-66 MOF structure as a carrier for fosfomycin antibiotic and also as an osteogenic differentiation promoter when introduced in 3D chitosan scaffolds, for the first time. Our results revealed that the wet-spun chitosan scaffolds containing fosfomycin loaded UiO-66 nanocrystals (CHI/UiO-66/FOS) possessed fiber mesh structure with integrated micro-scale fibers and increased mechanical strength. In vitro antibacterial studies indicated that CHI/UiO-66/FOS scaffolds showed bactericidal activity against Staphylococcus aureus. Moreover, the scaffolds were biocompatible to MC3T3-E1 pre-osteoblasts and significantly up-regulated the expression of osteogenesis-related genes and facilitated the extracellular matrix mineralization, in vitro. Taken together, our results demonstrate UiO-66 MOFs can present double functionality and CHI/UiO-66/FOS scaffolds hold a significant potential to be further explored as an alternative approach in treating infected bone defects like osteomyelitis.
|
| 巻・号 |
136
|
| ページ |
212757
|
| 公開日 |
2022-5-1
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| DOI |
10.1016/j.bioadv.2022.212757
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| PII |
S2772-9508(22)00034-6
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| PMID |
35929303
|
| MeSH |
Anti-Bacterial Agents / pharmacology
Chitosan* / chemistry
Fosfomycin* / pharmacology
Metal-Organic Frameworks* / pharmacology
Osteogenesis / genetics
Phthalic Acids
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
