論文 - 詳細
| RRC ID | 73688 |
|---|---|
| 著者 | Shishiuchi R, Kang H, Tagami T, Ueda Y, Lang W, Kimura A, Okuyama M. |
| タイトル | Discovery of α-l-Glucosidase Raises the Possibility of α-l-Glucosides in Nature. |
| ジャーナル | ACS Omega |
| Abstract |
Glucose, a common monosaccharide in nature, is dominated by the d-enantiomer. Meanwhile, the discovery of l-glucose-utilizing bacteria and the elucidation of their metabolic pathways 10 years ago suggests that l-glucose exists naturally. Most carbohydrates exist as glycosides rather than monosaccharides; therefore, we expected that nature also contains l-glucosides. Sequence analysis within glycoside hydrolase family 29 led us to identify two α-l-glucosidases, ClAgl29A and ClAgl29B, derived from Cecembia lonarensis LW9. ClAgl29A and ClAgl29B exhibited higher Km, kcat, and kcat/Km values for p-nitrophenyl α-l-glucoside than that for p-nitrophenyl α-l-fucoside. Structural analysis of ClAgl29B in complex with l-glucose showed that these enzymes have an active-site pocket that preferentially binds α-l-glucoside, but excludes α-l-fucoside. These results suggest that ClAgl29A and ClAgl29B evolved to hydrolyze α-l-glucoside, implying the existence of α-l-glucoside in nature. Furthermore, α-l-glucosidic linkages (α-l-Glc-(1 → 3)-l-Glc, α-l-Glc-(1 → 2)-l-Glc, and α-l-Glc-(1 → 6)-l-Glc) were synthesized by the transglucosylation activity of ClAgl29A and ClAgl29B. We believe that this study will lead to new research on α-l-glucosides, including determining the physiological effects on humans, and the discovery of novel α-l-glucoside-related enzymes. |
| 巻・号 | 7(50) |
| ページ | 47411-47423 |
| 公開日 | 2022-12-20 |
| DOI | 10.1021/acsomega.2c06991 |
| PMID | 36570207 |
| PMC | PMC9774334 |
| IF | 2.87 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Genomic DNA of Coraliomargarita akajimensis JCM 23193 T (JGD12235) |
| 一般微生物 | JCM 23193 |