論文 - 詳細
| RRC ID | 73743 |
|---|---|
| 著者 | Matsuo K, Abiko Y, Yamano S, Matsusue K, Kumagai Y. |
| タイトル | Activation of HSP90/HSF1 Signaling as an Adaptive Response to an Electrophilic Metabolite of Morphine. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
Morphinone (MO) is an electrophilic metabolite of morphine that covalently binds to protein thiols, resulting in toxicity in vitro and in vivo. We have previously identified a variety of redox signaling pathways that are activated during electrophilic stress. However, the role of MO in such activation remains unknown. In this study, we examined whether MO could activate heat shock protein (HSP) 90/heat shock factor (HSF) 1 signaling in HepG2 cells. MO exposure caused S-modification of HSP90 (determined using biotin-PEAC5-maleimide labeling) and nuclear translocation of transcription factor HSF1, thereby up-regulating its downstream genes encoding B-cell lymphoma 2-associated anthanogene 3 and heat shock 70 kDa protein 1. However, dihydromorphinone, a non-electrophilic metabolite of morphine, had little effect on HSF1 activation or upregulation of these genes, suggesting that covalent modification plays a role in this process and that the HSP90/HSF1 pathway is a redox-signaled adaptive response to morphine metabolism. |
| 巻・号 | 46(2) |
| ページ | 334-337 |
| 公開日 | 2023-1-1 |
| DOI | 10.1248/bpb.b22-00531 |
| PMID | 36724961 |
| MeSH | DNA-Binding Proteins* / genetics DNA-Binding Proteins* / metabolism HSP70 Heat-Shock Proteins / genetics HSP90 Heat-Shock Proteins Heat Shock Transcription Factors / genetics Hep G2 Cells Humans Morphine* / pharmacology Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 1.863 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1886) |