RRC ID 73751
Author Davì V, Tanimoto H, Ershov D, Haupt A, De Belly H, Le Borgne R, Couturier E, Boudaoud A, Minc N.
Title Mechanosensation Dynamically Coordinates Polar Growth and Cell Wall Assembly to Promote Cell Survival.
Journal Dev Cell
Abstract How growing cells cope with size expansion while ensuring mechanical integrity is not known. In walled cells, such as those of microbes and plants, growth and viability are both supported by a thin and rigid encasing cell wall (CW). We deciphered the dynamic mechanisms controlling wall surface assembly during cell growth, using a sub-resolution microscopy approach to monitor CW thickness in live rod-shaped fission yeast cells. We found that polar cell growth yielded wall thinning and that thickness negatively influenced growth. Thickness at growing tips exhibited a fluctuating behavior with thickening phases followed by thinning phases, indicative of a delayed feedback promoting thickness homeostasis. This feedback was mediated by mechanosensing through the CW integrity pathway, which probes strain in the wall to adjust synthase localization and activity to surface growth. Mutants defective in thickness homeostasis lysed by rupturing the wall, demonstrating its pivotal role for walled cell survival.
Volume 45(2)
Pages 170-182.e7
Published 2018-4-23
DOI 10.1016/j.devcel.2018.03.022
PII S1534-5807(18)30242-9
PMID 29689193
MeSH Biomechanical Phenomena Cell Cycle Cell Polarity Cell Proliferation Cell Shape Cell Survival Cell Wall / physiology* Cell Wall / ultrastructure Models, Biological Morphogenesis / physiology* Schizosaccharomyces / physiology* Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / metabolism* Stress, Mechanical
IF 10.092