論文 - 詳細
| RRC ID | 73752 |
|---|---|
| 著者 | Okamura K, Ishizuka A, Siomi H, Siomi MC. |
| タイトル | Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. |
| ジャーナル | Genes Dev |
| Abstract |
In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA-induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. In Drosophila, Argonaute2 (AGO2) was identified as part of the RISC complex. Here we show that AGO2 is an essential component for siRNA-directed RNA interference (RNAi) response and is required for the unwinding of siRNA duplex and in consequence assembly of siRNA into RISC in Drosophila embryos. However, Drosophila embryos lacking AGO2, which are siRNA-directed RNAi-defective, are still capable of miRNA-directed target RNA cleavage. In contrast, Argonaute1 (AGO1), another Argonaute protein in fly, which is dispensable for siRNA-directed target RNA cleavage, is required for mature miRNA production that impacts on miRNA-directed RNA cleavage. The association of AGO1 with Dicer-1 and pre-miRNA also suggests that AGO1 is involved in miRNA biogenesis. Our findings show that distinct Argonaute proteins act at different steps of the small RNA silencing mechanism and suggest that there are inherent differences between siRNA-initiated RISCs and miRNA-initiated RISCs in Drosophila. |
| 巻・号 | 18(14) |
| ページ | 1655-66 |
| 公開日 | 2004-7-15 |
| DOI | 10.1101/gad.1210204 |
| PII | 1210204 |
| PMID | 15231716 |
| PMC | PMC478188 |
| MeSH | Animals Argonaute Proteins Base Sequence Blotting, Northern DNA Primers Drosophila / embryology* Drosophila Proteins / metabolism* Drosophila Proteins / physiology Embryo, Nonmammalian / metabolism Eukaryotic Initiation Factors MicroRNAs / biosynthesis* RNA Interference / physiology* RNA, Messenger / metabolism* RNA, Small Interfering / metabolism* RNA-Induced Silencing Complex / metabolism* RNA-Induced Silencing Complex / physiology |
| IF | 9.527 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ショウジョウバエ | 10001Ab-1 |