論文 - 詳細
| RRC ID | 73763 |
|---|---|
| 著者 | Lu K, Wazawa T, Sakamoto J, Vu CQ, Nakano M, Kamei Y, Nagai T. |
| タイトル | Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer. |
| ジャーナル | Nano Lett |
| Abstract |
Despite improved sensitivity of nanothermometers, direct observation of heat transport inside single cells has remained challenging for the lack of high-speed temperature imaging techniques. Here, we identified insufficient temperature resolution under short signal integration time and slow sensor kinetics as two major bottlenecks. To overcome the limitations, we developed B-gTEMP, a nanothermometer based on the tandem fusion of mNeonGreen and tdTomato fluorescent proteins. We visualized the propagation of heat inside intracellular space by tracking the temporal variation of local temperature at a time resolution of 155 μs and a temperature resolution 0.042 °C. By comparing the fast in situ temperature dynamics with computer-simulated heat diffusion, we estimated the thermal diffusivity of live HeLa cells. The present thermal diffusivity in cells was about 1/5.3 of that of water and much smaller than the values reported for bulk tissues, which may account for observations of heterogeneous intracellular temperature distributions. |
| 巻・号 | 22(14) |
| ページ | 5698-5707 |
| 公開日 | 2022-7-27 |
| DOI | 10.1021/acs.nanolett.2c00608 |
| PMID | 35792763 |
| PMC | PMC9335883 |
| MeSH | HeLa Cells Hot Temperature* Humans Temperature Water* |
| IF | 11.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 36 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | B-gTEMP/pRSET-b (RDB19859) B-gTEMP/pcDNA3 (RDB19860) |