論文 - 詳細
| RRC ID | 73795 |
|---|---|
| 著者 | Tanaka S. |
| タイトル | Interaction of replication factor Sld3 and histone acetyl transferase Esa1 alleviates gene silencing and promotes the activation of late and dormant replication origins. |
| ジャーナル | Genetics |
| Abstract |
DNA replication in eukaryotes is a multi-step process that consists of three main reactions: helicase loading (licensing), helicase activation (firing), and nascent DNA synthesis (elongation). Although the contributions of some chromatin regulatory factors in the licensing and elongation reaction have been determined, their functions in the firing reaction remain elusive. In the budding yeast Saccharomyces cerevisiae, Sld3, Sld7, and Cdc45 (3-7-45) are rate-limiting in the firing reaction and simultaneous overexpression of 3-7-45 causes untimely activation of late and dormant replication origins. Here, we found that 3-7-45 overexpression not only activated dormant origins in the silenced locus, HMLα, but also exerted an anti-silencing effect at this locus. For these, interaction between Sld3 and Esa1, a conserved histone acetyltransferase, was responsible. Moreover, the Sld3-Esa1 interaction was required for the untimely activation of late origins. These results reveal the Sld3-Esa1 interaction as a novel level of regulation in the firing reaction. |
| 巻・号 | 217(1) |
| ページ | 1-11 |
| 公開日 | 2021-3-3 |
| DOI | 10.1093/genetics/iyaa001 |
| PII | 5974037 |
| PMID | 33683348 |
| PMC | PMC8045687 |
| MeSH | Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation, Fungal* Gene Silencing* Histone Acetyltransferases / genetics Histone Acetyltransferases / metabolism* Replication Origin Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* |
| IF | 4.015 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BY29137 BY29147 BY29148 BY29149 BY29138 BY29145 BY29146 |