論文 - 詳細
| RRC ID | 73814 |
|---|---|
| 著者 | Yoshimoto S, Suzuki T, Otani N, Takahashi D, Toshima K, Dohmae N, Simizu S. |
| タイトル | Destabilization of vitelline membrane outer layer protein 1 homolog (VMO1) by C-mannosylation. |
| ジャーナル | FEBS Open Bio |
| Abstract |
C-mannosylation is a rare type of protein glycosylation whereby a single mannose is added to the first tryptophan in the consensus sequence Trp-Xaa-Xaa-Trp/Cys (in which Xaa represents any amino acid). Its consensus sequence is mainly found in proteins containing a thrombospondin type-1 repeat (TSR1) domain and in type I cytokine receptors. In these proteins, C-mannosylation affects protein secretion, intracellular localization, and protein stability; however, the role of C-mannosylation in proteins that are not type I cytokine receptors and/or do not contain a TSR1 domain is less well explored. In this study, we focused on human vitelline membrane outer layer protein 1 homolog (VMO1). VMO1, which possesses two putative C-mannosylation sites, is a 21-kDa secreted protein that does not contain a TSR1 domain and is not a type I cytokine receptor. Mass spectrometry analyses revealed that VMO1 is C-mannosylated at Trp105 but not at Trp44 . Although C-mannosylation does not affect the extracellular secretion of VMO1, it destabilizes the intracellular VMO1. In addition, a structural comparison between VMO1 and C-mannosylated VMO1 showed that the modification of the mannose changes the conformation of three loops in VMO1. Taken together, our results demonstrate the first example of C-mannosylation for protein destabilization of VMO1. |
| 公開日 | 2023-1-20 |
| DOI | 10.1002/2211-5463.13561 |
| PMID | 36680395 |
| PMC | PMC9989928 |
| MeSH | Glycosylation Humans Mannose* / metabolism Protein Transport Receptors, Cytokine / metabolism Vitelline Membrane* / metabolism |
| IF | 2.231 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |
| 遺伝子材料 | CSII-CMV-MCS-IRES2-Bsd (RDB04385) VMO1-GH (RDB20014) VMO1-MH (RDB20015) VMO1/W105F-MH (RDB20016) |