Reference - Detail
| RRC ID | 73836 |
|---|---|
| Author | Matos-Perdomo E, Santana-Sosa S, Ayra-Plasencia J, Medina-Suárez S, Machín F. |
| Title | The vacuole shapes the nucleus and the ribosomal DNA loop during mitotic delays. |
| Journal | Life Sci Alliance |
| Abstract |
The ribosomal DNA (rDNA) array of Saccharomyces cerevisiae has served as a model to address chromosome organization. In cells arrested before anaphase (mid-M), the rDNA acquires a highly structured chromosomal organization referred to as the rDNA loop, whose length can double the cell diameter. Previous works established that complexes such as condensin and cohesin are essential to attain this structure. Here, we report that the rDNA loop adopts distinct presentations that arise as spatial adaptations to changes in the nuclear morphology triggered during mid-M arrests. Interestingly, the formation of the rDNA loop results in the appearance of a space under the loop (SUL) which is devoid of nuclear components yet colocalizes with the vacuole. We show that the rDNA-associated nuclear envelope (NE) often reshapes into a ladle to accommodate the vacuole in the SUL, with the nucleus becoming bilobed and doughnut-shaped. Finally, we demonstrate that the formation of the rDNA loop and the SUL require TORC1, membrane synthesis and functional vacuoles, yet is independent of nucleus-vacuole junctions and rDNA-NE tethering. |
| Volume | 5(10) |
| Published | 2022-10-1 |
| DOI | 10.26508/lsa.202101161 |
| PII | 5/10/e202101161 |
| PMID | 35961781 |
| PMC | PMC9375157 |
| MeSH | Anaphase DNA, Ribosomal / genetics DNA, Ribosomal / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins* / genetics Saccharomyces cerevisiae Proteins* / metabolism Vacuoles* / metabolism |
| IF | 2.622 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 25 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | BYP6849(pMK42) BYP6850(pMK72) BY25598(YNK54) |