論文 - 詳細
| RRC ID | 73851 |
|---|---|
| 著者 | Fujie N, Ito M, Kishida M, Hirata Y, Kondo A, Tanaka T. |
| タイトル | Metabolic engineering of Schizosaccharomyces pombe for itaconic acid production. |
| ジャーナル | J Biotechnol |
| Abstract |
The economical production of value-added chemicals from renewable biomass is a promising aspect of producing a sustainable economy. Itaconic acid (IA) is a high value-added compound that is expected to be an alternative to petroleum-based chemicals. In this study, we developed a metabolic engineering strategy for the large-scale production of IA from glucose using the fission yeast Schizosaccharomyces pombe. Heterologous expression of the cis-aconitic acid decarboxylase (CAD) gene from Aspergillus terreus, which encodes cis-aconitate decarboxylase in the cytosol, led to the production of 0.132 g/L of IA. We demonstrated that mitochondrial localization of CAD enhanced the production of IA. To prevent the leakage of carbon flux from the TCA cycle, we generated a strain in which the endogenous malate exporter, citrate lyase, and citrate transporter genes were disrupted. A titer of 1.110 g/L of IA was obtained from a culture of this strain started with 50 g/L of glucose. By culturing the multiple mutant strain at increased cell density, we succeeded in enhancing the IA production to 1.555 g/L. The metabolic engineering strategies presented in this study have the potential to improve the titer of the biosynthesis of derivatives of intermediates of the TCA cycle. |
| 巻・号 | 358 |
| ページ | 111-117 |
| 公開日 | 2022-11-10 |
| DOI | 10.1016/j.jbiotec.2022.09.006 |
| PII | S0168-1656(22)00215-2 |
| PMID | 36122598 |
| MeSH | Aconitic Acid / metabolism Carboxy-Lyases* / genetics Carboxy-Lyases* / metabolism Glucose / metabolism Malates Metabolic Engineering / methods Petroleum* Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Succinates / metabolism |
| IF | 3.503 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY12804 |