論文 - 詳細
| RRC ID | 73911 |
|---|---|
| 著者 | Morita K, Seike T, Ishii J, Matsuda F, Shimizu H. |
| タイトル | Improvement of 2,3-butanediol production by dCas9 gene expression system in Saccharomyces cerevisiae. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Saccharomyces cerevisiae has been widely used in bioproduction. To produce a target product other than ethanol, ethanol production must be decreased to enhance target production. An ethanol non-producing yeast strain was previously constructed by knocking out pyruvate decarboxylase (PDC) genes in the ethanol synthetic pathway. However, glucose uptake by the ethanol-non-producing yeast strain was significantly decreased. In this study, dead Cas9 (dCas9) was used to reduce ethanol synthesis during 2,3-butanediol production without reduction of glucose. The binding site of guide RNA used to effectively suppress PDC1 promoter-driven red fluorescent protein expression by dCas9 was identified and applied to control PDC1 expression. The production of 2,3-butanediol rather than ethanol was improved in repetitive test tube culture. Additionally, ethanol production was decreased and 2,3-butanediol production was increased in the strain expressing dCas9 targeting the PDC1 promoter in the third round of cultivation, compared with the control strain. |
| 巻・号 | 133(3) |
| ページ | 208-212 |
| 公開日 | 2022-3-1 |
| DOI | 10.1016/j.jbiosc.2021.12.007 |
| PII | S1389-1723(21)00334-0 |
| PMID | 34998687 |
| MeSH | Butylene Glycols / metabolism Gene Expression Pyruvate Decarboxylase / genetics Saccharomyces cerevisiae* / genetics Saccharomyces cerevisiae* / metabolism Saccharomyces cerevisiae Proteins* / genetics Saccharomyces cerevisiae Proteins* / metabolism |
| IF | 2.366 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | pGK424 |