論文 - 詳細
| RRC ID | 73946 |
|---|---|
| 著者 | Inouye S, Noguchi M, Sakaki Y, Takagi Y, Miyata T, Iwanaga S, Miyata T, Tsuji FI. |
| タイトル | Cloning and sequence analysis of cDNA for the luminescent protein aequorin. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
The luminescent jellyfish Aequorea contains a photoprotein, aequorin, which emits light by an intramolecular reaction in the presence of a trace amount of Ca2+. A cDNA library of Aequorea was constructed and clones carrying the cDNA for the Ca2+-dependent photoprotein were isolated by the method of colony hybridization using synthetic oligonucleotide probes. The primary structure of the protein deduced from the nucleotide sequence showed that the protein is composed of 189 amino acid residues and has three E-F hand structures that are characteristic for Ca2+-binding sites. The sequence also suggested that the protein has hydrophobic regions at which the protein may interact with its functional chromophore, coelenterazine. |
| 巻・号 | 82(10) |
| ページ | 3154-8 |
| 公開日 | 1985-5-1 |
| DOI | 10.1073/pnas.82.10.3154 |
| PMID | 3858813 |
| PMC | PMC397733 |
| MeSH | Aequorin / genetics* Amino Acid Sequence Apoproteins / genetics Base Sequence Binding Sites Calcium / metabolism Cloning, Molecular DNA / genetics Luminescent Measurements Luminescent Proteins / genetics* Protein Conformation |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 68 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | pJN-opAQ (RDB19830) pAQ-B (RDB19836) |