RRC ID 73946
Author Inouye S, Noguchi M, Sakaki Y, Takagi Y, Miyata T, Iwanaga S, Miyata T, Tsuji FI.
Title Cloning and sequence analysis of cDNA for the luminescent protein aequorin.
Journal Proc Natl Acad Sci U S A
Abstract The luminescent jellyfish Aequorea contains a photoprotein, aequorin, which emits light by an intramolecular reaction in the presence of a trace amount of Ca2+. A cDNA library of Aequorea was constructed and clones carrying the cDNA for the Ca2+-dependent photoprotein were isolated by the method of colony hybridization using synthetic oligonucleotide probes. The primary structure of the protein deduced from the nucleotide sequence showed that the protein is composed of 189 amino acid residues and has three E-F hand structures that are characteristic for Ca2+-binding sites. The sequence also suggested that the protein has hydrophobic regions at which the protein may interact with its functional chromophore, coelenterazine.
Volume 82(10)
Pages 3154-8
Published 1985-5-1
DOI 10.1073/pnas.82.10.3154
PMID 3858813
PMC PMC397733
MeSH Aequorin / genetics* Amino Acid Sequence Apoproteins / genetics Base Sequence Binding Sites Calcium / metabolism Cloning, Molecular DNA / genetics Luminescent Measurements Luminescent Proteins / genetics* Protein Conformation
IF 9.412
DNA material pJN-opAQ (RDB19830) pAQ-B (RDB19836)