Reference - Detail
| RRC ID | 73947 |
|---|---|
| Author | Inouye S. |
| Title | NAD(P)H-flavin oxidoreductase from the bioluminescent bacterium, Vibrio fischeri ATCC 7744, is a flavoprotein. |
| Journal | FEBS Lett |
| Abstract |
The NAD(P)H-flavin oxidoreductase gene from the bioluminescent bacterium, Vibrio fischeri ATCC 7744, was expressed in Escherichia coli, and the enzyme purified using Cibacron Blue 3G-A affinity column chromatography from crude extracts in a single step. The purified enzyme had a typical flavoprotein absorption spectrum and flavin mononucleotide (FMN) was identified as a prosthetic group, non-covalently bound in a molar ratio of 1:1. The enzyme catalyzed the electron transfer from NADH via FMNH2 to various other electron acceptors. Reduced flavin produced by flavin reductase participated non-enzymatically in the following reactions: H2O2-forming NADH oxidase-like, oxygen-insensitive nitroreductase-like, diaphorase (quinone reductase)-like and bacterial luciferase reactions. |
| Volume | 347(2-3) |
| Pages | 163-8 |
| Published | 1994-6-27 |
| DOI | 10.1016/0014-5793(94)00528-1 |
| PII | 0014-5793(94)00528-1 |
| PMID | 8033996 |
| MeSH | Amino Acid Sequence Chromatography, Affinity Electron Transport Electrophoresis, Polyacrylamide Gel Enzyme Stability Escherichia coli FMN Reductase Flavin Mononucleotide / analysis Flavin Mononucleotide / chemistry Flavin Mononucleotide / metabolism Hot Temperature Hydrogen Peroxide / metabolism Hydrogen-Ion Concentration Luminescence Molecular Sequence Data Molecular Weight NAD / metabolism NADH, NADPH Oxidoreductases / chemistry NADH, NADPH Oxidoreductases / genetics NADH, NADPH Oxidoreductases / metabolism* Recombinant Proteins Spectrophotometry Vibrio / enzymology* |
| IF | 3.057 |
| Resource | |
| DNA material | pJN-FRase (RDB19835) |