Reference - Detail
| RRC ID | 73948 |
|---|---|
| Author | Inouye S, Tsuji FI. |
| Title | Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. |
| Journal | FEBS Lett |
| Abstract |
Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein. |
| Volume | 341(2-3) |
| Pages | 277-80 |
| Published | 1994-3-21 |
| DOI | 10.1016/0014-5793(94)80472-9 |
| PII | 0014-5793(94)80472-9 |
| PMID | 8137953 |
| MeSH | Amino Acid Sequence Animals Base Sequence DNA, Complementary Fluorescence Green Fluorescent Proteins Luminescent Proteins / chemistry Luminescent Proteins / genetics* Molecular Sequence Data Recombinant Proteins / chemistry Recombinant Proteins / genetics Scyphozoa / genetics* Sequence Alignment |
| IF | 3.057 |
| Resource | |
| DNA material | pAGP (RDB19839) |