RRC ID 73948
Author Inouye S, Tsuji FI.
Title Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein.
Journal FEBS Lett
Abstract Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.
Volume 341(2-3)
Pages 277-80
Published 1994-3-21
DOI 10.1016/0014-5793(94)80472-9
PII 0014-5793(94)80472-9
PMID 8137953
MeSH Amino Acid Sequence Animals Base Sequence DNA, Complementary Fluorescence Green Fluorescent Proteins Luminescent Proteins / chemistry Luminescent Proteins / genetics* Molecular Sequence Data Recombinant Proteins / chemistry Recombinant Proteins / genetics Scyphozoa / genetics* Sequence Alignment
IF 3.057
DNA material pAGP (RDB19839)