RRC ID 73954
Author Inouye S, Sahara Y.
Title Expression, purification and characterization of a photoprotein, clytin, from Clytia gregarium.
Journal Protein Expr Purif
Abstract A novel histidine-tagged secretion vector in Escherichia coli was constructed and large amounts of highly pure clytin, a calcium-binding photoprotein, was prepared. The histidine-tagged apoclytin expressed into the periplasmic space in E. coli was purified by nickel chelate affinity chromatography. Recombinant clytin was regenerated from apoclytin by incubation with coelenterazine in the presence of dithiothreitol and then purified by anion-exchange chromatography and hydrophobic chromatography. The yield of recombinant clytin was 20mg from 2L of cultured cells with purity greater than 95%. Luminescence properties of recombinant clytin were identical to that of native clytin (phialidin). The Ca(2+) sensitivity of recombinant clytin is lower than that of aequorin and clytin is suited for measuring higher concentration of Ca(2+). Semi-synthetic clytins were also prepared with coelenterazine analogues, and the initial intensity, luminescence capacity and half decay time were characterized.
Volume 53(2)
Pages 384-9
Published 2007-6-1
DOI 10.1016/j.pep.2006.12.014
PII S1046-5928(06)00413-X
PMID 17275329
MeSH Amino Acid Sequence Animals Base Sequence Calcium / pharmacology DNA Primers / genetics Escherichia coli / genetics Gene Expression Genetic Vectors Hydrozoa / genetics* Luminescent Measurements Luminescent Proteins / genetics* Luminescent Proteins / isolation & purification* Molecular Sequence Data Plasmids / genetics Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Spectrophotometry
IF 1.513
DNA material piP-H-XEL (RDB19856)