RRC ID 73957
Author Inouye S, Sahara Y.
Title Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system.
Journal Biochem Biophys Res Commun
Abstract We constructed a cold induced expression vector in Escherichia coli cells that consists of a histidine tag sequence for nickel chelate affinity purification, IgG-binding domain of protein A (ZZ-domain) and the multiple cloning sites. The role of ZZ-domain as a solubilizing partner at 15 degrees C was demonstrated by expressing the imidazopyrazinone-type luciferases of Renilla, Oplophorus, Gaussia, and Vargula (Cypridina) as well as the calcium-binding photoproteins and firefly luciferase. The fused protein with ZZ-domain was expressed efficiently as a soluble form in the cytoplasm of E. coli cells at low temperature.
Volume 376(3)
Pages 448-53
Published 2008-11-21
DOI 10.1016/j.bbrc.2008.08.149
PII S0006-291X(08)01684-7
PMID 18789309
MeSH Aequorin / biosynthesis Aequorin / chemistry Aequorin / genetics Amino Acid Sequence Animals Apoproteins / biosynthesis Apoproteins / chemistry Apoproteins / genetics CCAAT-Enhancer-Binding Proteins / genetics Cold Temperature* Escherichia coli / genetics Genetic Vectors* Heat-Shock Proteins / genetics Humans Immunoglobulin G / immunology Luciferases / genetics Molecular Sequence Data Protein Biosynthesis* Protein Structure, Tertiary / genetics Recombinant Fusion Proteins / biosynthesis* Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Proteins / biosynthesis Recombinant Proteins / chemistry Recombinant Proteins / genetics Solubility Staphylococcal Protein A / chemistry Staphylococcal Protein A / genetics Staphylococcal Protein A / immunology
IF 2.985
DNA material pJN-ZZ (RDB19844)