論文 - 詳細
| RRC ID | 73959 |
|---|---|
| 著者 | Inouye S, Sato J, Sasaki S, Sahara Y. |
| タイトル | Streptavidin-aequorin fusion protein for bioluminescent immunoassay. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
The fusion protein of streptavidin to aequorin (STA-AQ) was highly purified from inclusion bodies in Escherichia coli cells and applied to a bioluminescent sandwich immunoassay. α-Fetoprotein (AFP), which is a serological marker of liver cancer, was used as a model analyte to test STA-AQ in an immunoassay. The measurable range of AFP by the sandwich immunoassay, using the complex of STA-AQ and the biotinylated anti-AFP antibody, was 0.02-200 ng/mL with an average coefficient of variation of 4.9%. The detection sensitivity with the complex of STA-AQ and the biotinylated anti-AFP antibody was similar to that with the complex of biotinylated aequorin, streptavidin and the biotinylated anti-AFP antibody. STA-AQ would be a useful reporter protein for immunoassays. |
| 巻・号 | 75(3) |
| ページ | 568-71 |
| 公開日 | 2011-1-1 |
| DOI | 10.1271/bbb.100798 |
| PII | JST.JSTAGE/bbb/100798 |
| PMID | 21389603 |
| MeSH | Aequorin / genetics Aequorin / metabolism* Antibodies / chemistry Antibodies / immunology Antibodies / metabolism Biomarkers, Tumor / analysis* Biomarkers, Tumor / immunology Biotinylation Calcium / metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli Gene Expression Humans Immunoassay Light Limit of Detection Liver Neoplasms / diagnosis Luminescent Measurements Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism* Streptavidin / genetics Streptavidin / metabolism* Streptomyces / genetics Streptomyces / metabolism alpha-Fetoproteins / analysis* alpha-Fetoproteins / immunology |
| IF | 1.516 |
| リソース情報 | |
| 遺伝子材料 | pSTA51 (RDB19843) |