論文 - 詳細
| RRC ID | 73960 |
|---|---|
| 著者 | Suzuki T, Kondo C, Kanamori T, Inouye S. |
| タイトル | Video rate bioluminescence imaging of secretory proteins in living cells: localization, secretory frequency, and quantification. |
| ジャーナル | Anal Biochem |
| Abstract |
We have developed a method of video rate bioluminescence imaging to investigate protein secretion from a single mammalian cell and analyzed the localization, secretory frequency, and quantification of secreted protein. By detecting the luminescence signals from the Gaussia luciferase (GLase) reaction using a high-speed electron-multiplying charge-coupled device (EM-CCD) camera, video rate imaging was performed with a time resolution within 500 ms/image over 30 min in living cells. As a model study, we applied the method to visualize the glucose-stimulated insulin secretion from clustered pancreatic MIN6 β cells using the fused protein of GLase with preproinsulin. High-quality video images clearly showed that the glucose-stimulated insulin secretion from the clustered MIN6 β cells oscillated within a period of a few minutes over 10 min. In addition, the glibenclamide-induced insulin secretion from the clustered MIN6 β cells was visualized, suggesting that bioluminescence video rate imaging is a useful method for validating drug action in living cells. |
| 巻・号 | 415(2) |
| ページ | 182-9 |
| 公開日 | 2011-8-15 |
| DOI | 10.1016/j.ab.2011.03.039 |
| PII | S0003-2697(11)00226-0 |
| PMID | 21477579 |
| MeSH | Animals Copepoda / enzymology Glucose / pharmacology Glyburide / pharmacology Imidazoles / chemistry Imidazoles / pharmacology Insulin / analysis* Insulin / genetics Insulin / metabolism Insulin Secretion Insulin-Secreting Cells / metabolism* Luciferases / genetics Luciferases / metabolism Luminescent Measurements / methods* Mice Protein Precursors / genetics Protein Precursors / metabolism Pyrazines / chemistry Pyrazines / pharmacology Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Video Recording / methods* |
| IF | 2.877 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pJNC-hINS-GLuc (RDB19845) |