Reference - Detail
| RRC ID | 73964 |
|---|---|
| Author | Inouye S, Suzuki T. |
| Title | Protein expression of preferred human codon-optimized Gaussia luciferase genes with an artificial open-reading frame in mammalian and bacterial cells. |
| Journal | Protein Expr Purif |
| Abstract |
The protein expressions of three preferred human codon-optimized Gaussia luciferase genes (pGLuc, EpGLuc, and KpGLuc) were characterized in mammalian and bacterial cells by comparing them with those of wild-type Gaussia luciferase gene (wGLuc) and human codon-optimized Gaussia luciferase gene (hGLuc). Two synthetic genes of EpGLuc and KpGLuc containing the complete preferred human codons have an artificial open-reading frame; however, they had the similar protein expression levels to those of pGLuc and hGLuc in mammalian cells. In bacterial cells, the protein expressions of pGLuc, EpGLuc, and KpGLuc with approximately 65% GC content were the same and showed approximately 60% activities of wGLuc and hGLuc. The artificial open-reading frame in EpGLuc and KpGLuc did not affect the protein expression in mammalian and bacterial cells. |
| Volume | 128 |
| Pages | 93-100 |
| Published | 2016-12-1 |
| DOI | 10.1016/j.pep.2016.08.006 |
| PII | S1046-5928(16)30154-1 |
| PMID | 27506135 |
| MeSH | Animals CHO Cells Cricetinae Cricetulus Escherichia coli / genetics Escherichia coli / metabolism* Gene Expression* Humans Luciferases* / biosynthesis Luciferases* / genetics Open Reading Frames* Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Silent Mutation* |
| IF | 1.513 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pJNC-pGLuc (RDB19855) |