| Abstract |
There are various reports on embryogenesis in silkworm, Bombyx mori, a model organism for lepidopteran insects. New tissue observation methods have been developed with the development of biological science. Applying these methods to silkworm eggs makes it possible to capture morphological and histological features that have not been observed until now. Tissue transparency technology is a method of observation that has advanced remarkably recently. This study emphasized the CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) method. The tissue clearing reagent used in CUBIC is water-soluble, containing urea and amino alcohol, which easily and effectively clears animal tissue by immersing the sample in the reagent. This study showed that silkworm eggs are made transparent using the modified CUBIC method at the fertilization stage. Furthermore, we observed the behavior of egg nucleus, polar body nuclei, and sperm nuclei at the fertilization stage.
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