Reference - Detail
| RRC ID | 74145 |
|---|---|
| Author | Nerome K, Imagawa T, Sugita S, Arasaki Y, Maegawa K, Kawasaki K, Tanaka T, Watanabe S, Nishimura H, Suzuki T, Kuroda K, Kosugi I, Kajiura Z. |
| Title | The potential of a universal influenza virus-like particle vaccine expressing a chimeric cytokine. |
| Journal | Life Sci Alliance |
| Abstract |
The efficacy of the current influenza vaccines is frequently reduced because of antigenic drift, a trade-off of developing improved vaccines with broad cross-protective activity against influenza A viruses. In this study, we have successfully constructed a chimeric cytokine (CC) comprising the M2 protein, influenza A neuraminidase stalk, and interleukin-12. We produced virus-like particles (VLPs) containing CC and influenza hemagglutinin (HA) proteins using a baculovirus system in Eri silkworm pupae. The protective efficacy of the CCHA-VLP vaccine was evaluated in mice. The CCFkH5HA-VLP vaccine increased the survival rates of BALB/c mice, infected with a lethal dose of PRH1 and HKH5 viruses, to 80% and 100%, respectively. The results suggested that CCHA-VLP successfully induced potent cross-reactive protective immunity against infection with homologous and heterologous subtypes of the influenza A virus. This is the first study to design a CC-containing HA-VLP vaccine and validate its protective efficacy. |
| Volume | 6(1) |
| Published | 2022-11-7 |
| DOI | 10.26508/lsa.202201548 |
| PII | 6/1/e202201548 |
| PMID | 36344085 |
| PMC | PMC9644419 |
| MeSH | Animals Antibodies, Viral Cytokines Hemagglutinins Humans Influenza Vaccines* Influenza, Human* / prevention & control Mice Mice, Inbred BALB C Orthomyxoviridae Infections* / prevention & control Vaccines, Virus-Like Particle* / genetics |
| IF | 2.622 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | Eri silkworm |