| RRC ID |
74197
|
| 著者 |
Yamano S, Tsukuda Y, Mizuhara N, Yamaguchi Y, Ogita A, Fujita KI.
|
| タイトル |
Dehydrozingerone enhances the fungicidal activity of glabridin against Saccharomyces cerevisiae and Candida albicans.
|
| ジャーナル |
Lett Appl Microbiol
|
| Abstract |
Drug resistance commonly occurs when treating immunocompromized patients with fungal infections. Dehydrozingerone-a phenolic compound isolated from the rhizome of Zingiber officinale-inhibits drug efflux in Saccharomyces cerevisiae by overexpression of the ATP-binding cassette (ABC) transporter Pdr5p. We aimed to investigate whether dehydrozingerone enhances the antifungal activity of glabridin-an isoflavan isolated from the roots of Glycyrrhiza glabra L.-by attenuating multidrug resistance through the intrinsic expression system of multidrug-efflux-related genes in a wild-type strain of the model yeast. The antifungal activity of 50 μmol l-1 glabridin alone was weak and temporary against S. cerevisiae; however, cell viability was significantly inhibited when the cells were co-treated with glabridin and dehydrozingerone. This enhancement was also observed in human pathogenic Candida albicans. Glabridin efflux did not depend on a particular drug efflux pump; instead, the transcription factors PDR1 and PDR3-regulating the transcription of multiple genes encoding drug efflux pumps-were involved in the antifungal activity and efflux of glabridin. qRT-PCR analysis revealed that dehydrozingerone reduced glabridin-induced overexpression of the ABC transporter-related genes PDR1, PDR3, and PDR5 to the levels observed in untreated cells. Our findings indicated that dehydrozingerone potentiates the efficacy of plant-derived antifungals through its effects on ABC transporters.
|
| 巻・号 |
76(4)
|
| 公開日 |
2023-4-3
|
| DOI |
10.1093/lambio/ovad040
|
| PII |
7093404
|
| PMID |
36990694
|
| MeSH |
ATP-Binding Cassette Transporters / genetics
Antifungal Agents / metabolism
Antifungal Agents / pharmacology
Candida albicans
Fungal Proteins / genetics
Humans
Saccharomyces cerevisiae* / metabolism
Saccharomyces cerevisiae Proteins* / genetics
|
| IF |
2.173
|
| リソース情報 |
| 酵母 |
BY25929 |
