論文 - 詳細
| RRC ID | 74207 |
|---|---|
| 著者 | Adisorn Ratanayotha, Makoto Matsuda, Yukiko Kimura, Fumiko Takenaga, Tomoaki Mizuno, Md. Israil Hossain, Shin-ichi Higashijima, Takafumi Kawai, Michio Ogasawara, Yasushi Okamura |
| タイトル | Voltage-sensing phosphatase (Vsp) regulates endocytosis-dependent nutrient absorption in chordate enterocytes |
| ジャーナル | Communications Biology |
| Abstract |
Voltage-sensing phosphatase (Vsp) is a unique membrane protein that translates membrane electrical activities into the changes of phosphoinositide profiles. Vsp orthologs from various species have been intensively investigated toward their biophysical properties, primarily using a heterologous expression system. In contrast, the physiological role of Vsp in native tissues remains largely unknown. Here we report that zebrafish Vsp (Dr-Vsp), encoded by tpte gene, is functionally expressed on the endomembranes of lysosome-rich enterocytes (LREs) that mediate dietary protein absorption via endocytosis in the zebrafish mid-intestine. Dr-Vsp-deficient LREs were remarkably defective in forming endosomal vacuoles after initial uptake of dextran and mCherry. Dr-Vsp-deficient zebrafish exhibited growth restriction and higher mortality during the critical period when zebrafish larvae rely primarily on exogenous feeding via intestinal absorption. Furthermore, our comparative study on marine invertebrate Ciona intestinalis Vsp (Ci-Vsp) revealed co-expression with endocytosis-associated genes in absorptive epithelial cells of the Ciona digestive tract, corresponding to zebrafish LREs. These findings signify a crucial role of Vsp in regulating endocytosis-dependent nutrient absorption in specialized enterocytes across animal species. |
| 巻・号 | 5 |
| 公開日 | 2022-9-10 |
| DOI | 10.1038/s42003-022-03916-6 |
| PMID | 36088390 |
| PMC | PMC9464190 |
| MeSH | Animals Ciona intestinalis* Endocytosis Enterocytes / metabolism Nutrients Phosphoric Monoester Hydrolases* / genetics Zebrafish / genetics Zebrafish / metabolism |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(tpte(vsp):GFP) |