| RRC ID |
74243
|
| Author |
Maeda H, Ichimizu S, Watanabe H, Hamasaki K, Chikamatsu M, Murata R, Yumoto N, Seki T, Katsuki H, Otagiri M, Maruyama T.
|
| Title |
Cell-penetrating albumin enhances the sublingual delivery of antigens through macropinocytosis.
|
| Journal |
Int J Biol Macromol
|
| Abstract |
Innovations in oral immunotherapy have greatly advanced the therapeutic control of allergies. However, these therapeutic effects suffer from the fact that the amount of antigen delivered to antigen-presenting cells is limited given the formulations that are currently available. We recently designed a cell-penetrating albumin and found that this modified albumin enters cells via the induction of macropinocytosis. Herein, we report on a novel system for delivering antigens based on cell-penetrating albumin-inducible macropinocytosis that allows larger amounts of antigens to be delivered to antigen-presenting cells. A treatment with cell-penetrating albumin significantly increased the permeability of ovalbumin (45 kDa) or dextran (2000 kDa) on monolayers derived from human oral squamous carcinoma cells. Flow cytometric analyses showed that the cell-penetrating albumin treatment resulted in a significant elevation in the amount of dextran that was delivered to two types of antigen-presenting cells. Finally, mice that had been sensitized by Japanese cedar pollen extract (JCPE) and cell-penetrating albumin showed a decline in the frequency of nose-rubbing against a subsequent intranasal administration of JCPE. These findings suggest that the sublingual administration of cell-penetrating albumin efficiently delivers antigens to antigen-presenting cells via the induction of macropinocytosis, resulting in an enhancement in the therapeutic effect of sublingual immunotherapy.
|
| Volume |
221
|
| Pages |
1439-1452
|
| Published |
2022-11-30
|
| DOI |
10.1016/j.ijbiomac.2022.09.132
|
| PII |
S0141-8130(22)02063-3
|
| PMID |
36126807
|
| MeSH |
Administration, Sublingual
Allergens
Animals
Antigens
Dextrans*
Humans
Hypersensitivity*
Mice
Ovalbumin
|
| IF |
5.162
|
| Resource |
| Human and Animal Cells |
THP-1 |
