論文 - 詳細
| RRC ID | 74299 |
|---|---|
| 著者 | Takeuchi T, Hata T, Miyanishi H, Yuasa T, Setoguchi S, Takeda A, Morimoto N, Hikima JI, Sakai M, Kono T. |
| タイトル | Diel rhythm of the inflammatory cytokine il1b in the Japanese medaka (Oryzias latipes) regulated by core components of the circadian clock. |
| ジャーナル | Fish Shellfish Immunol |
| Abstract |
In recent years, studies on circadian control in immunity have been actively conducted in mammals, but little is known about circadian rhythms in the field of fish immunology. In this study, we aimed to analyse the regulation of the diel oscillation of inflammatory cytokine interleukin-1β (il1b) gene expression by core components of the circadian clock in Japanese medaka (Oryzias latipes). The expression of il1b and clock genes (bmal1 and clock1) in medaka acclimated to a 12:12 light (L): dark (D) cycle showed diel rhythm. Additionally, higher expression of il1b was detected in medaka embryo cells (OLHdrR-e3) overexpressing bmal1 and clock1. A significant decrease in il1b expression was observed in OLHdrR-e3 cells after bmal1 knockdown using morpholino oligos. These changes may be mediated by transcriptional regulation via clock proteins, which target the E-box sequence in the cis-element of il1b as identified using luciferase reporter assays. Moreover, LPS stimulation and pathogenic bacterial infection at different zeitgeber time (ZT) under LD12:12 conditions affected the degree of il1b expression, which showed high and low responsiveness to both immuno-stimulations at ZT2 and ZT14, respectively. These results suggested that fish IL-1β exhibited diel oscillation regulated by clock proteins, and its responsiveness to immune-stimulation depends on the time of day. |
| 巻・号 | 127 |
| ページ | 238-246 |
| 公開日 | 2022-8-1 |
| DOI | 10.1016/j.fsi.2022.06.020 |
| PII | S1050-4648(22)00338-2 |
| PMID | 35724845 |
| MeSH | ARNTL Transcription Factors / genetics ARNTL Transcription Factors / metabolism Animals CLOCK Proteins / genetics CLOCK Proteins / metabolism Circadian Clocks* / genetics Circadian Rhythm / genetics Cytokines / metabolism Interleukin-1beta / genetics Interleukin-1beta / metabolism Mammals / metabolism Oryzias* / genetics Oryzias* / metabolism |
| IF | 3.298 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | Saito (WS249) |