Reference - Detail
| RRC ID | 74301 |
|---|---|
| Author | Li X, Li X, Li W, Zhang Y, Guo H, Wang G, Li Y, Wu X, Hu R, Wang S, Zhao X, Chen L, Guan G. |
| Title | Sex-specific meiosis responses to Gsdf in medaka (Oryzias latipes). |
| Journal | FEBS J |
| Abstract |
The meiotic entry of undifferentiated germ cells is sexually specific and strictly regulated by the testicular or ovarian environment. Germline stem cells with a set of abnormal sex chromosomes and associated autosomes undergo defective meiotic processes and are eventually eliminated by yet to be defined post-transcriptional modifications. Herein, we report the role of gsdf, a member of BMP/TGFβ family uniquely found in teleost, in the regulation of meiotic entry in medaka (Oryzias latipes) via analyses of gametogenesis in gsdf-deficient XX and XY gonads in comparison with their wild-type siblings. Several differentially expressed genes, including the FKB506-binding protein 7 (fkbp7), were significantly upregulated in pubertal gsdf-deficient gonads. The increase in alternative pre-mRNA isoforms of meiotic synaptonemal complex gene sycp3 was visualized using Integrative Genomics Viewer and confirmed by real-time qPCR. Nevertheless, immunofluorescence analysis showed that Sycp3 protein products reduced significantly in gsdf-deficient XY oocytes. Transmission electron microscope observations showed that normal synchronous cysts were replaced by asynchronous cysts in gsdf-deficient testis. Breeding experiments showed that the sex ratio deviation of gsdf-/- XY gametes in a non-Mendelian manner might be due to the non-segregation of XY chromosomes. Taken together, our results suggest that gsdf plays a role in the proper execution of cytoplasmic and nuclear events through receptor Smad phosphorylation and Sycp3 dephosphorylation to coordinate medaka gametogenesis, including sex-specific mitotic divisions and meiotic recombination. |
| Published | 2022-12-14 |
| DOI | 10.1111/febs.16701 |
| PMID | 36515005 |
| IF | 4.392 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Peer-review(Publons) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Medaka | HdrR-II1(IB178) HNI-II(IB176) |