論文 - 詳細
| RRC ID | 74321 |
|---|---|
| 著者 | Liu W, Ota M, Tabushi M, Takahashi Y, Takakura Y. |
| タイトル | Development of allergic rhinitis immunotherapy using antigen-loaded small extracellular vesicles. |
| ジャーナル | J Control Release |
| Abstract |
Allergic rhinitis is caused by a breakdown of the Th1/Th2 balance, in which the allergen-induced Th2 immune response predominates over the Th1 immune response, culminating in IgE-mediated anaphylaxis. In this study, we used small extracellular vesicles (sEVs), cell-derived membrane vesicles with a particle size of 100 nm, as simultaneous delivery carriers for allergens (ovalbumin, OVA) and CpG DNA, an adjuvant that can induce a Th1 immune response, for the treatment of allergic rhinitis. sEVs loaded with CpG DNA and OVA(CpG-OVA-sEVs) were successfully prepared. CpG-OVA-sEVs possessed an average particle size of 90 nm and average zeta potential of -30 mV. CpG DNA modification did not influence the uptake of sEVs by dendritic cells and CpG-OVA-sEV can activate dendritic cells. The CpG-OVA-sEVs were delivered to the nasopharynx-associated lymphoid tissue (NALT) of mice and were primarily taken up by the CD11c positive cells after intranasal administration. Intranasally administering CpG-OVA-sEVs significantly enhanced OVA-specific IgG antibody titers in mice models of allergic rhinitis, suggesting a transformed Th1/2 balance. Moreover, The CpG-OVA-sEV administration alleviated allergic symptoms compared to the control group. Further, the amount of IgE secreted in mouse serum decreased. Thus, CpG-OVA-sEVs could be a useful therapeutic method for treating allergic rhinitis. |
| 巻・号 | 345 |
| ページ | 433-442 |
| 公開日 | 2022-5-1 |
| DOI | 10.1016/j.jconrel.2022.03.016 |
| PII | S0168-3659(22)00142-0 |
| PMID | 35301052 |
| MeSH | Allergens Animals Cytokines DNA / therapeutic use Disease Models, Animal Extracellular Vesicles* / metabolism Immunoglobulin E Immunotherapy Mice Mice, Inbred BALB C Ovalbumin Rhinitis, Allergic* / drug therapy Th2 Cells |
| IF | 7.727 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16/BL6(RCB2638) |