論文 - 詳細
| RRC ID | 74351 |
|---|---|
| 著者 | Agathe Couturier, Chloé Virolle, Kelly Goldlust, Annick Berne-Dedieu, Audrey Reuter, Sophie Nolivos, Yoshiharu Yamaichi, Sarah Bigot, Christian Lesterlin |
| タイトル | Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer |
| ジャーナル | Nature Communications |
| Abstract |
Conjugation is a contact-dependent mechanism for the transfer of plasmid DNA between bacterial cells, which contributes to the dissemination of antibiotic resistance. Here, we use live-cell microscopy to visualise the intracellular dynamics of conjugative transfer of F-plasmid in E. coli, in real time. We show that the transfer of plasmid in single-stranded form (ssDNA) and its subsequent conversion into double-stranded DNA (dsDNA) are fast and efficient processes that occur with specific timing and subcellular localisation. Notably, the ssDNA-to-dsDNA conversion determines the timing of plasmid-encoded protein production. The leading region that first enters the recipient cell carries single-stranded promoters that allow the early and transient synthesis of leading proteins immediately upon entry of the ssDNA plasmid. The subsequent conversion into dsDNA turns off leading gene expression, and activates the expression of other plasmid genes under the control of conventional double-stranded promoters. This molecular strategy allows for the timely production of factors sequentially involved in establishing, maintaining and disseminating the plasmid. |
| 巻・号 | 14 |
| 公開日 | 2023-1-18 |
| DOI | 10.1038/s41467-023-35978-3 |
| PMID | 36653393 |
| PMC | PMC9849209 |
| MeSH | Conjugation, Genetic* DNA DNA, Bacterial / genetics DNA, Bacterial / metabolism DNA, Single-Stranded / genetics Escherichia coli* / genetics Escherichia coli* / metabolism Gene Transfer, Horizontal Plasmids / genetics |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 137 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | MG1655 |