論文 - 詳細
| RRC ID | 74371 |
|---|---|
| 著者 | Masatoshi Miyakoshi, Teppei Morita, Asaki Kobayashi, Anna Berger, Hiroki Takahashi, Yasuhiro Gotoh, Tetsuya Hayashi, Kan Tanaka |
| タイトル | Glutamine synthetase mRNA releases sRNA from its 3′UTR to regulate carbon/nitrogen metabolic balance in Enterobacteriaceae |
| ジャーナル | eLife |
| Abstract |
Glutamine synthetase (GS) is the key enzyme of nitrogen assimilation induced under nitrogen limiting conditions. The carbon skeleton of glutamate and glutamine, 2-oxoglutarate, is supplied from the TCA cycle, but how this metabolic flow is controlled in response to nitrogen availability remains unknown. We show that the expression of the E1o component of 2-oxoglutarate dehydrogenase, SucA, is repressed under nitrogen limitation in Salmonella enterica and Escherichia coli. The repression is exerted at the post-transcriptional level by an Hfq-dependent sRNA GlnZ generated from the 3′UTR of the GS-encoding glnA mRNA. Enterobacterial GlnZ variants contain a conserved seed sequence and primarily regulate sucA through base-pairing far upstream of the translation initiation region. During growth on glutamine as the nitrogen source, the glnA 3′UTR deletion mutants expressed SucA at higher levels than the S. enterica and E. coli wild-type strains, respectively. In E. coli, the transcriptional regulator Nac also participates in the repression of sucA. Lastly, this study clarifies that the release of GlnZ from the glnA mRNA by RNase E is essential for the post-transcriptional regulation of sucA. Thus, the mRNA coordinates the two independent functions to balance the supply and demand of the fundamental metabolites. |
| 巻・号 | 11 |
| 公開日 | 2022-11-28 |
| DOI | 10.7554/elife.82411 |
| PMID | 36440827 |
| PMC | PMC9731577 |
| MeSH | 3' Untranslated Regions Carbon* Enterobacteriaceae Escherichia coli / genetics Glutamate-Ammonia Ligase / genetics Glutamine / genetics Nitrogen* RNA, Messenger / genetics |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062 |