| RRC ID |
74378
|
| 著者 |
Haruka Nasu, Riko Shirakawa, Kazuyuki Furuta, Chikara Kaito
|
| タイトル |
Knockout of mlaA increases Escherichia coli virulence in a silkworm infection model
|
| ジャーナル |
PLOS ONE
|
| Abstract |
The mlaA gene encodes a lipoprotein to maintain an outer membrane lipid asymmetry in gram-negative bacteria. Although the role of mlaA in bacterial virulence has been studied in several bacterial species, there are no reports of its role in E. coli virulence. In this study, we found that knockout of mlaA in E. coli increased its virulence against silkworms. The mlaA-knockout mutant was sensitive to several antibiotics and detergents, but resistant to vancomycin and chlorhexidine. The mlaA-knockout mutant grew faster than the parent strain in the presence of silkworm hemolymph. The mlaA-knockout mutant also produced a larger amount of outer membrane vesicles than the parent strain. These findings suggest that mlaA knockout causes E. coli resistance to specific antimicrobial substances and increases outer membrane vesicle production, thereby enhancing E. coli virulence properties in the silkworm infection model.
|
| 巻・号 |
17
|
| ページ |
e0270166
|
| 公開日 |
2022-7-13
|
| DOI |
10.1371/journal.pone.0270166
|
| PMID |
35830444
|
| PMC |
PMC9278758
|
| MeSH |
Animals
Bacterial Outer Membrane Proteins / genetics
Bombyx* / genetics
Escherichia coli / genetics
Escherichia coli Infections*
Escherichia coli Proteins* / genetics
Virulence / genetics
|
| IF |
2.74
|
| リソース情報 |
| 原核生物(大腸菌) |
JD23323
JW3794
JW0617
JW2343 |
