RRC ID 74427
Author Nakayama M, Matsushita F, Hama C.
Title The matrix protein Hikaru genki localizes to cholinergic synaptic clefts and regulates postsynaptic organization in the Drosophila brain.
Journal J Neurosci
Abstract The synaptic cleft, a crucial space involved in neurotransmission, is filled with extracellular matrix that serves as a scaffold for synaptic differentiation. However, little is known about the proteins present in the matrix and their functions in synaptogenesis, especially in the CNS. Here, we report that Hikaru genki (Hig), a secreted protein with an Ig motif and complement control protein domains, localizes specifically to the synaptic clefts of cholinergic synapses in the Drosophila CNS. The data indicate that this specific localization is achieved by capture of secreted Hig in synaptic clefts, even when it is ectopically expressed in glia. In the absence of Hig, the cytoskeletal scaffold protein DLG accumulated abnormally in cholinergic postsynapses, and the synaptic distribution of acetylcholine receptor (AchR) subunits Dα6 and Dα7 significantly decreased. hig mutant flies consistently exhibited resistance to the AchR agonist spinosad, which causes lethality by specifically activating the Dα6 subunit, suggesting that loss of Hig compromises the cholinergic synaptic activity mediated by Dα6. These results indicate that Hig is a specific component of the synaptic cleft matrix of cholinergic synapses and regulates their postsynaptic organization in the CNS.
Volume 34(42)
Pages 13872-7
Published 2014-10-15
DOI 10.1523/JNEUROSCI.1585-14.2014
PII 34/42/13872
PMID 25319684
PMC PMC6705295
MeSH Animals Animals, Genetically Modified Brain / physiology* Brain Chemistry / physiology Cholinergic Neurons / chemistry Cholinergic Neurons / physiology* Drosophila Drosophila Proteins / analysis Drosophila Proteins / physiology* Male Nerve Tissue Proteins / analysis Nerve Tissue Proteins / physiology* Post-Synaptic Density / chemistry Post-Synaptic Density / physiology* Synapses / chemistry Synapses / physiology*
IF 5.674
Drosophila DGRC118939 DGRC118940 DGRC118941