Reference - Detail
RRC ID | 74442 |
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Author | Kobayashi Y, Watanabe N, Hiura R, Kubota M, Furuta K, Sugimoto K, Murota K, Nakamura E, Matsuura T, Kai K, Inui T, Kitakaze T, Harada N, Yamaji R. |
Title | Transport Form and Pathway from the Intestine to the Peripheral Tissues and the Intestinal Absorption and Metabolism Properties of Oleamide. |
Journal | J Agric Food Chem |
Abstract |
This study aimed to obtain information on the transport form and pathway from the intestine to the peripheral tissues and on the intestinal absorption and metabolism properties of oleamide (cis-9-octadecenamide). Oleamide was primarily transported via the portal vein. Density gradient centrifugation indicated that plasma oleamide was enriched in the fractions containing albumin in the portal and peripheral blood. Oleamide formed a complex with albumin in an endothermic reaction (apparent Kd = 4.4 μM). The CD36 inhibitor inhibited the oleamide uptake into the intestinal epithelial Caco-2 cells, and oleamide decreased the cell surface CD36 level. The fatty acid amide hydrolase (FAAH) inhibitor increased the transepithelial transport of oleamide across Caco-2 cells and the plasma oleamide concentration in mice intragastrically administered with oleamide. These results indicate that oleamide is transported primarily via the portal vein as a complex with albumin. Furthermore, we suggest that oleamide is taken up via CD36 in the small intestine and degraded intracellularly by FAAH. |
Volume | 70(49) |
Pages | 15499-15508 |
Published | 2022-12-14 |
DOI | 10.1021/acs.jafc.2c06791 |
PMID | 36458736 |
MeSH | Albumins Animals Caco-2 Cells Humans Intestinal Absorption* Intestine, Small* Mice |
IF | 4.192 |
Resource | |
Human and Animal Cells | CACO-2(RCB0988) |