| RRC ID |
74491
|
| Author |
Uchiyama H, Muramatsu D, Higashi H, Kida H, Iwai A.
|
| Title |
Effects of chondroitin sulfate oligosaccharides on osteoclast differentiation of RAW264 cells, and myotube differentiation of C2C12 cells.
|
| Journal |
PLoS One
|
| Abstract |
Chondroitin sulfate (CS) is a glycosaminoglycan, and CS derived from various animal species is used in drugs and food supplements to alleviate arthralgia. The CS is a high molecular weight compound, and hydrolysis of CS by intestinal microbiota is thought to be required for absorption in mammalians. Chondroitin sulfate oligosaccharides (Oligo-CS) are produced by hydrolysis with subcritical water from CS isolated from a species of skate, Raja pulchra for the improvement of bioavailability. The present study conducted in vitro experiments using murine cell lines, to compare the biological activities of Oligo-CS and high molecular weight CS composed with the similar disaccharide isomer units of D-glucuronic acid and N-acetyl-D-glucosamine (CS-C). The results show that Oligo-CS inhibits osteoclast differentiation of RAW264 cells significantly at lower concentrations than in CS. The cell viability of a myoblast cell line, C2C12 cells, was increased when the cells were grown in a differentiated medium for myotubes with Oligo-CS, where there were no effects on the cell viability in CS. These results suggest that in vitro Oligo-CS exhibits stronger bioactivity than high-molecular weight CS.
|
| Volume |
18(4)
|
| Pages |
e0284343
|
| Published |
2023-1-1
|
| DOI |
10.1371/journal.pone.0284343
|
| PII |
PONE-D-22-32503
|
| PMID |
37053208
|
| PMC |
PMC10101473
|
| MeSH |
Animals
Cell Differentiation
Chondroitin Sulfates* / metabolism
Chondroitin Sulfates* / pharmacology
Mammals / metabolism
Mice
Muscle Fibers, Skeletal / metabolism
Oligosaccharides / pharmacology
Osteoclasts* / metabolism
|
| IF |
2.74
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
