論文 - 詳細
| RRC ID | 74499 |
|---|---|
| 著者 | Hoedjes KM, Kostic H, Flatt T, Keller L. |
| タイトル | A Single Nucleotide Variant in the PPARγ-homolog Eip75B Affects Fecundity in Drosophila. |
| ジャーナル | Mol Biol Evol |
| Abstract |
Single nucleotide polymorphisms are the most common type of genetic variation, but how these variants contribute to the adaptation of complex phenotypes is largely unknown. Experimental evolution and genome-wide association studies have demonstrated that variation in the PPARγ-homolog Eip75B has associated with longevity and life-history differences in the fruit fly Drosophila melanogaster. Using RNAi knockdown, we first demonstrate that reduced expression of Eip75B in adult flies affects lifespan, egg-laying rate, and egg volume. We then tested the effects of a naturally occurring SNP within a cis-regulatory domain of Eip75B by applying two complementary approaches: a Mendelian randomization approach using lines of the Drosophila Genetic Reference Panel, and allelic replacement using precise CRISPR/Cas9-induced genome editing. Our experiments reveal that this natural polymorphism has a significant pleiotropic effect on fecundity and egg-to-adult viability, but not on longevity or other life-history traits. Our results provide a rare functional validation at the nucleotide level and identify a natural allelic variant affecting fitness and life-history adaptation. |
| 巻・号 | 40(2) |
| 公開日 | 2023-2-3 |
| DOI | 10.1093/molbev/msad018 |
| PII | 7005670 |
| PMID | 36703226 |
| PMC | PMC9922802 |
| MeSH | Animals DNA-Binding Proteins* / genetics Drosophila / genetics Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Fertility / genetics Genome-Wide Association Study Longevity / genetics Nucleotides / metabolism PPAR gamma / genetics PPAR gamma / metabolism Polymorphism, Single Nucleotide Transcription Factors / metabolism |
| IF | 11.062 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | CAS-0001 |