| RRC ID |
74501
|
| 著者 |
Cabrera AJH, Gumbiner BM, Kwon YV.
|
| タイトル |
Remodeling of E-cadherin subcellular localization during cell dissemination.
|
| ジャーナル |
Mol Biol Cell
|
| Abstract |
Given the role of E-cadherin (E-cad) in holding epithelial cells together, an inverse relationship between E-cad levels and cell invasion during the epithelial-mesenchymal transition and cancer metastasis has been well recognized. Here we report that E-cad is necessary for the invasiveness of RasV12-transformed intestinal epithelial cells in Drosophila. E-cad/β-catenin disassembles at adherens junctions and assembles at invasive protrusions--the actin- and cortactin-rich invadopodium-like protrusions associated with the breach of the extracellular matrix (ECM)--during dissemination of RasV12-transformed intestinal epithelial cells. Loss of E-cad impairs the elongation of invasive protrusions and attenuates the ability of RasV12-transformed cells to compromise the ECM. Notably, E-cad and cortactin affect each other's localization to invasive protrusions. Given the essential roles of cortactin in cell invasion, our observations indicate that E-cad plays a role in the invasiveness of RasV12-transformed intestinal epithelial cells by controlling cortactin localization to invasive protrusions. Thus our study demonstrates that E-cad is a component of invasive protrusions and provides molecular insights into the unconventional role of E-cad in cell dissemination in vivo.
|
| 巻・号 |
34(5)
|
| ページ |
ar46
|
| 公開日 |
2023-5-1
|
| DOI |
10.1091/mbc.E23-03-0087
|
| PMID |
36989029
|
| MeSH |
Actins / metabolism
Adherens Junctions / metabolism
Animals
Cadherins* / metabolism
Cortactin* / metabolism
Drosophila / metabolism
Epithelial Cells / metabolism
|
| IF |
3.791
|
| リソース情報 |
| ショウジョウバエ |
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