論文 - 詳細
| RRC ID | 74508 |
|---|---|
| 著者 | Tang HW, Spirohn K, Hu Y, Hao T, Kovács IA, Gao Y, Binari R, Yang-Zhou D, Wan KH, Bader JS, Balcha D, Bian W, Booth BW, Coté AG, de Rouck S, Desbuleux A, Goh KY, Kim DK, Knapp JJ, Lee WX, Lemmens I, Li C, Li M, Li R, Lim HJ, Liu Y, Luck K, Markey D, Pollis C, Rangarajan S, Rodiger J, Schlabach S, Shen Y, Sheykhkarimli D, TeeKing B, Roth FP, Tavernier J, Calderwood MA, Hill DE, Celniker SE, Vidal M, Perrimon N, Mohr SE. |
| タイトル | Next-generation large-scale binary protein interaction network for Drosophila melanogaster. |
| ジャーナル | Nat Commun |
| Abstract |
Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of > 10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of data from FlyBi and previous PPI studies using an orthogonal assay allows for normalization of data quality; subsequent integration of FlyBi and previous data results in an expanded binary Drosophila reference interaction network, DroRI, comprising 17,232 interactions among 6511 proteins. We use FlyBi data to generate an autophagy network, then validate in vivo using autophagy-related assays. The deformed wings (dwg) gene encodes a protein that is both a regulator and a target of autophagy. Altogether, these resources provide a foundation for building new hypotheses regarding protein networks and function. |
| 巻・号 | 14(1) |
| ページ | 2162 |
| 公開日 | 2023-4-15 |
| DOI | 10.1038/s41467-023-37876-0 |
| PII | 10.1038/s41467-023-37876-0 |
| PMID | 37061542 |
| PMC | PMC10105736 |
| MeSH | Animals Drosophila / genetics Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Protein Interaction Mapping / methods Protein Interaction Maps* / genetics Saccharomyces cerevisiae / metabolism Two-Hybrid System Techniques |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 27 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 11523R-4 12576R-1 14655R-1 31807R-2 4393R-4 4496R-3 4813R-1 5059R-1 5059R-3 5089R-1 5104R-1 5674R-1 7006R-2 7101R-1 8159R-3 17051R-3 HMJ22430 2711R-1 9241R-2 4973R-1 3424R-1 9573R-1 9218R-1 5352R-3, |