論文 - 詳細
| RRC ID | 74528 |
|---|---|
| 著者 | Blaby-Haas CE, Padilla-Benavides T, Stübe R, Argüello JM, Merchant SS. |
| タイトル | Evolution of a plant-specific copper chaperone family for chloroplast copper homeostasis. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Metallochaperones traffic copper (Cu(+)) from its point of entry at the plasma membrane to its destination. In plants, one destination is the chloroplast, which houses plastocyanin, a Cu-dependent electron transfer protein involved in photosynthesis. We present a previously unidentified Cu(+) chaperone that evolved early in the plant lineage by an alternative-splicing event of the pre-mRNA encoding the chloroplast P-type ATPase in Arabidopsis 1 (PAA1). In several land plants, recent duplication events created a separate chaperone-encoding gene coincident with loss of alternative splicing. The plant-specific Cu(+) chaperone delivers Cu(+) with specificity for PAA1, which is flipped in the envelope relative to prototypical bacterial ATPases, compatible with a role in Cu(+) import into the stroma and consistent with the canonical catalytic mechanism of these enzymes. The ubiquity of the chaperone suggests conservation of this Cu(+)-delivery mechanism and provides a unique snapshot into the evolution of a Cu(+) distribution pathway. We also provide evidence for an interaction between PAA2, the Cu(+)-ATPase in thylakoids, and the Cu(+)-chaperone for Cu/Zn superoxide dismutase (CCS), uncovering a Cu(+) network that has evolved to fine-tune Cu(+) distribution. |
| 巻・号 | 111(50) |
| ページ | E5480-7 |
| 公開日 | 2014-12-16 |
| DOI | 10.1073/pnas.1421545111 |
| PII | 1421545111 |
| PMID | 25468978 |
| PMC | PMC4273408 |
| MeSH | Arabidopsis / genetics* Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Chlamydomonas reinhardtii / genetics Chloroplast Proton-Translocating ATPases / metabolism Chloroplasts / metabolism Chloroplasts / physiology* Cloning, Molecular Computational Biology Copper / metabolism* Evolution, Molecular* Homeostasis / physiology* Immunoblotting Metallochaperones / genetics* Metallochaperones / metabolism Superoxide Dismutase / metabolism |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda19661 pda04914 |