RRC ID 74571
Author Fang K, Murakami Y, Kanda S, Shimono T, Dang AT, Ono M, Nishiyama T.
Title Unkeito Suppresses RANKL-Mediated Osteoclastogenesis via the Blimp1-Bcl6 and NF-κB Signaling Pathways and Enhancing Osteoclast Apoptosis.
Journal Int J Mol Sci
Abstract Osteoporosis is a common bone disease, particularly in menopausal women. Herein, we screened four Kampo medicines (Unkeito (UKT), Kamishoyosan (KSS), Kamikihito (KKT), and Ninjinyoeito (NYT)), frequently used to treat menopausal syndromes, for their effects on receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclast differentiation in RAW 264 cells. Considering that UKT exhibited the most potent effect, we examined its effect on RANKL-induced osteoclastogenesis, the induction of osteoclast apoptosis, and the mechanisms underlying its effects. UKT inhibits RANKL-induced osteoclast differentiation in the early stage and decreases osteoclast-related genes, including tartrate-resistant acid phosphatase (Trap), dendritic cell-specific transmembrane protein (Dcstamp), matrix metalloproteinase-9 (Mmp9), and cathepsin K (Ctsk). Specifically, UKT inhibits the nuclear factor of activated T cells 1 (NFATc1), which is essential for osteoclastogenesis. UKT increases Bcl6, which antagonizes NFATc1 and Dc-stamp, thereby blocking the progression of osteoclasts to maturation. UKT also decreased nuclear translocation by downregulating the activity of p65/NF-κB. In addition, UKT enhances mononuclear osteoclast apoptosis via activation of caspase-3. Herein, we demonstrate that UKT suppresses RANKL-mediated osteoclastogenesis via the Blimp1-Bcl6 and NF-κB signaling pathways and enhances mononuclear osteoclast apoptosis. Furthermore, UKT prevents bone loss in OVX mice. Thus, UKT might be a potential therapeutic agent for postmenopausal osteoporosis.
Volume 23(14)
Published 2022-7-15
DOI 10.3390/ijms23147814
PII ijms23147814
PMID 35887169
PMC PMC9323376
MeSH Animals Apoptosis Bone Resorption* / metabolism Cell Differentiation Female Humans Mice NF-kappa B / metabolism NFATC Transcription Factors / metabolism Osteoclasts* / metabolism Osteogenesis Positive Regulatory Domain I-Binding Factor 1 / metabolism Proto-Oncogene Proteins c-bcl-6 RANK Ligand / metabolism RANK Ligand / pharmacology Signal Transduction
IF 4.556
Human and Animal Cells RAW 264(RCB0535)