| RRC ID |
74595
|
| Author |
Liang Y, Furukawa H, Sakamoto K, Inaba H, Matsuura K.
|
| Title |
Anticancer Activity of Reconstituted Ribonuclease S-Decorated Artificial Viral Capsid.
|
| Journal |
Chembiochem
|
| Abstract |
Ribonuclease S (RNase S) is an enzyme that exhibits anticancer activity by degrading RNAs within cancer cells; however, the cellular uptake efficiency is low due to its small molecular size. Here we generated RNase S-decorated artificial viral capsids with a size of 70-170 nm by self-assembly of the β-annulus-S-peptide followed by reconstitution with S-protein at neutral pH. The RNase S-decorated artificial viral capsids are efficiently taken up by HepG2 cells and exhibit higher RNA degradation activity in cells compared with RNase S alone. Cell viability assays revealed that RNase S-decorated capsids have high anticancer activity comparable to that of standard anticancer drugs.
|
| Volume |
23(15)
|
| Pages |
e202200220
|
| Published |
2022-8-3
|
| DOI |
10.1002/cbic.202200220
|
| PMID |
35676201
|
| PMC |
PMC9400862
|
| MeSH |
Capsid*
Capsid Proteins / chemistry
Peptides / chemistry
Ribonucleases* / pharmacology
|
| IF |
2.576
|
| Resource |
| Human and Animal Cells |
Hep G2 |
