Reference - Detail
| RRC ID | 74606 |
|---|---|
| Author | Ohsaka Y, Ohgiya S, Hoshino T, Ishizaki K. |
| Title | Mitochondrial genome-encoded ATPase subunit 6+8 mRNA increases in human hepatoblastoma cells in response to nonfatal cold stress. |
| Journal | Cryobiology |
| Abstract |
Cellular responses to cold stress have not been well clarified, compared with heat shock responses, especially in mammalian cells. We investigated cold-stress responses in human hepatoblastoma cells (HepG2) exposed to a nonfatal temperature of 17 degrees C. Under the condition, RNA and protein syntheses in the cells were highly, but incompletely, depressed and cell growth was impaired. A cDNA subtraction method was used to isolate mRNAs for which the levels were increased in cold-stressed cells compared with cells cultured at 37 degrees C. A transcript isolated by the screening was identified as ATPase subunit 6+8 mRNA that encodes components of a mitochondrial ATPase complex and that is transcribed from a mitochondrial genome. The copy number of the mitochondrial genome in cells was not changed by cold stress. Thus, HepG2 cells were treated with various concentrations of actinomycin D and chloramphenicol to assess the effects of transcriptional and translational reduction on the increased level of the ATPase subunit 6+8 mRNA. The mRNA level was increased in cells treated with low concentrations of the RNA or protein synthesis inhibitors. These results indicate that the increase in ATPase subunit 6+8 mRNA stimulated by cold stress could be mediated by a partial decline of transcription and/or translation in the cells. In addition, the degradation of ATPase subunit 6+8 mRNA was suppressed in cold-stressed cells compared with that in 37 degrees C-cultured cells. This result implies that posttranscriptional regulation is also involved in the cold-stimulated increase in ATPase subunit 6+8 mRNA in HepG2 cells. |
| Volume | 40(2) |
| Pages | 92-101 |
| Published | 2000-3-1 |
| DOI | 10.1006/cryo.2000.2237 |
| PII | S0011-2240(00)92237-5 |
| PMID | 10788308 |
| MeSH | Apoptosis Cold Temperature* DNA Replication DNA, Complementary / genetics DNA, Mitochondrial / genetics Enzyme Induction Gene Expression Regulation, Neoplastic* Hepatoblastoma / enzymology Hepatoblastoma / pathology* Humans Liver Neoplasms / enzymology Liver Neoplasms / pathology* Proton-Translocating ATPases / genetics* RNA, Messenger / biosynthesis* RNA, Neoplasm / biosynthesis* Stress, Physiological / genetics Stress, Physiological / metabolism Subtraction Technique Tumor Cells, Cultured / enzymology |
| IF | 2.287 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hep G2(RCB0459) |