| RRC ID |
74626
|
| 著者 |
Fujii S, Ohta T, Ehama R, Irikida M, Nomura S, Shoyama Y, Uto T.
|
| タイトル |
Development of an indirect competitive enzyme-linked immunosorbent assay for formononetin and its application in a cell-based assay using MC3T3-E1 cells.
|
| ジャーナル |
Food Chem
|
| Abstract |
Formononetin (FMN) is a methoxy isoflavone found abundantly in leguminous plants and associated foods. Several analytical methods have been developed to detect FMN. However, they are costly, complicated, and time-consuming. This study describes an indirect competitive enzyme-linked immunosorbent assay (icELISA) to determine FMN content in food samples using a monoclonal antibody (mAb) against FMN produced by a newly established hybridoma cell line. Validation studies were conducted, and this assay was found to be sufficiently reliable, with an analytical measurement range of 19.53-1250 ng/mL and a detection limit of 17.42 ng/mL. Furthermore, icELISA was successfully applied for a cell-based assay in which the amount of FMN and ononin uptake was quantified in MC3T3-E1 cells. Hence, icELISA is a simple and reliable method for the detection and quantification of FMN, as well as elucidation of its functions and underlying mechanisms of action.
|
| 巻・号 |
403
|
| ページ |
134339
|
| 公開日 |
2023-3-1
|
| DOI |
10.1016/j.foodchem.2022.134339
|
| PII |
S0308-8146(22)02301-9
|
| PMID |
36174344
|
| MeSH |
Animals
Enzyme-Linked Immunosorbent Assay / methods
Hybridomas / chemistry
Isoflavones* / analysis
Mice
Mice, Inbred BALB C
|
| IF |
6.306
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
