Reference - Detail
RRC ID | 74655 |
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Author | Schulz C. |
Title | Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster. |
Journal | Methods Mol Biol |
Abstract |
The Notch (N) signaling pathway plays versatile roles in development and disease of many model organisms (Andersson et al., Development 138, 3593-3612, 2011; Hori et al., J Cell Sci, 126, 2135-2140, 2013). However, studying a role for N in adult tissue of Drosophila melanogaster is challenging, because the gene is located on the X-chromosome. To reduce the expression of N specifically in either the germline or the somatic cells of the adult gonad, we used the CRISPR technology in combination with the UAS/Gal4 expression system. After generation of the flies, gonads were investigated for germline survival. Here, we outline our detailed protocol. |
Volume | 2472 |
Pages | 159-172 |
Published | 2022-1-1 |
DOI | 10.1007/978-1-0716-2201-8_13 |
PMID | 35674899 |
MeSH | Animals Clustered Regularly Interspaced Short Palindromic Repeats / genetics Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / metabolism Gonads / metabolism Male Receptors, Notch / genetics Receptors, Notch / metabolism Signal Transduction Technology |
Resource | |
Drosophila |