| RRC ID |
74673
|
| 著者 |
Yoshioka Y, Anzai K, Kowada R, Hiratsuka K, Hirayabu T, Yasuda M, Ohkawa Y, Sato T, Suyama M, Yoshida H, Yamaguchi M.
|
| タイトル |
Drosophila transcription factor NF-Y suppresses transcription of the lipase 4 gene, a key gene for lipid storage.
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| ジャーナル |
Exp Cell Res
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| Abstract |
The CCAAT motif-binding factor NF-Y consists of three different subunits, NF-YA, NF-YB, and NF-YC. Although it is suggested that NF-Y activity is essential for normal tissue homeostasis, survival, and metabolic function, its precise role in lipid metabolism is not clarified yet. In Drosophila, eye disc specific knockdown of Drosophila NF-YA (dNF-YA) induced aberrant morphology of the compound eye, the rough eye phenotype in adults and mutation of the lipase 4 (lip4) gene suppressed the rough eye phenotype. RNA-seq analyses with dNF-YA knockdown third instar larvae identified the lip4 gene as one of the genes that are up-regulated by the dNF-YA knockdown. We identified three dNF-Y-binding consensuses in the 5'flanking region of the lip4 gene, and a chromatin immunoprecipitation assay with the specific anti-dNF-YA IgG demonstrated dNF-Y binding to this genomic region. The luciferase transient expression assay with cultured Drosophila S2 cells and the lip4 promoter-luciferase fusion genes with and without mutations in the dNF-Y-binding consensuses showed that each of the three dNF-Y consensus sequences negatively regulated lip4 gene promoter activity. Consistent with these results, qRT-PCR analysis with the dNF-YA knockdown third instar larvae revealed that endogenous lip4 mRNA levels were increased by the knockdown of dNF-YA in vivo. The specific knockdown of dNF-YA in the fat body with the collagen-GAL4 driver resulted in smaller oil droplets in the fat body cells. Collectively, these results suggest that dNF-Y is involved in lipid storage through its negative regulation of lip4 gene transcription.
|
| 巻・号 |
420(1)
|
| ページ |
113307
|
| 公開日 |
2022-11-1
|
| DOI |
10.1016/j.yexcr.2022.113307
|
| PII |
S0014-4827(22)00300-7
|
| PMID |
36028059
|
| MeSH |
Animals
CCAAT-Binding Factor / genetics
CCAAT-Binding Factor / metabolism
Drosophila* / metabolism
Genes, vif
Immunoglobulin G / metabolism
Lipase / genetics
Lipase / metabolism
Lipids
Luciferases / metabolism
RNA, Messenger / metabolism
Transcription Factors* / metabolism
|
| IF |
3.383
|
| リソース情報 |
| ショウジョウバエ |
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