論文 - 詳細
| RRC ID | 74673 |
|---|---|
| 著者 | Yoshioka Y, Anzai K, Kowada R, Hiratsuka K, Hirayabu T, Yasuda M, Ohkawa Y, Sato T, Suyama M, Yoshida H, Yamaguchi M. |
| タイトル | Drosophila transcription factor NF-Y suppresses transcription of the lipase 4 gene, a key gene for lipid storage. |
| ジャーナル | Exp Cell Res |
| Abstract |
The CCAAT motif-binding factor NF-Y consists of three different subunits, NF-YA, NF-YB, and NF-YC. Although it is suggested that NF-Y activity is essential for normal tissue homeostasis, survival, and metabolic function, its precise role in lipid metabolism is not clarified yet. In Drosophila, eye disc specific knockdown of Drosophila NF-YA (dNF-YA) induced aberrant morphology of the compound eye, the rough eye phenotype in adults and mutation of the lipase 4 (lip4) gene suppressed the rough eye phenotype. RNA-seq analyses with dNF-YA knockdown third instar larvae identified the lip4 gene as one of the genes that are up-regulated by the dNF-YA knockdown. We identified three dNF-Y-binding consensuses in the 5'flanking region of the lip4 gene, and a chromatin immunoprecipitation assay with the specific anti-dNF-YA IgG demonstrated dNF-Y binding to this genomic region. The luciferase transient expression assay with cultured Drosophila S2 cells and the lip4 promoter-luciferase fusion genes with and without mutations in the dNF-Y-binding consensuses showed that each of the three dNF-Y consensus sequences negatively regulated lip4 gene promoter activity. Consistent with these results, qRT-PCR analysis with the dNF-YA knockdown third instar larvae revealed that endogenous lip4 mRNA levels were increased by the knockdown of dNF-YA in vivo. The specific knockdown of dNF-YA in the fat body with the collagen-GAL4 driver resulted in smaller oil droplets in the fat body cells. Collectively, these results suggest that dNF-Y is involved in lipid storage through its negative regulation of lip4 gene transcription. |
| 巻・号 | 420(1) |
| ページ | 113307 |
| 公開日 | 2022-11-1 |
| DOI | 10.1016/j.yexcr.2022.113307 |
| PII | S0014-4827(22)00300-7 |
| PMID | 36028059 |
| MeSH | Animals CCAAT-Binding Factor / genetics CCAAT-Binding Factor / metabolism Drosophila* / metabolism Genes, vif Immunoglobulin G / metabolism Lipase / genetics Lipase / metabolism Lipids Luciferases / metabolism RNA, Messenger / metabolism Transcription Factors* / metabolism |
| IF | 3.383 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |