Reference - Detail
| RRC ID | 74693 |
|---|---|
| Author | Chen J, St Johnston D. |
| Title | De novo apical domain formation inside the Drosophila adult midgut epithelium. |
| Journal | Elife |
| Abstract |
In the adult Drosophila midgut, basal intestinal stem cells give rise to enteroblasts that integrate into the epithelium as they differentiate into enterocytes. Integrating enteroblasts must generate a new apical domain and break through the septate junctions between neighbouring enterocytes, while maintaining barrier function. We observe that enteroblasts form an apical membrane initiation site (AMIS) when they reach the septate junction between the enterocytes. Cadherin clears from the apical surface and an apical space appears between above the enteroblast. New septate junctions then form laterally with the enterocytes and the AMIS develops into an apical domain below the enterocyte septate junction. The enteroblast therefore forms a pre-assembled apical compartment before it has a free apical surface in contact with the gut lumen. Finally, the enterocyte septate junction disassembles and the enteroblast/pre-enterocyte reaches the gut lumen with a fully formed brush border. The process of enteroblast integration resembles lumen formation in mammalian epithelial cysts, highlighting the similarities between the fly midgut and mammalian epithelia. |
| Volume | 11 |
| Published | 2022-9-28 |
| DOI | 10.7554/eLife.76366 |
| PII | 76366 |
| PMID | 36169289 |
| PMC | PMC9545526 |
| MeSH | Animals Cadherins Digestive System Drosophila* Drosophila Proteins* / genetics Drosophila melanogaster Epithelium Mammals |
| IF | 7.08 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#115611 DGRC#115111 DGRC#115514 DGRC#114660 |