RRC ID 74723
Author Khaitan V, Shill K, Chatterjee P, Mukherjee S, Majumder P.
Title Singed and vinculin play redundant roles in cell migration by regulating F-actin.
Journal Dev Dyn
Abstract INTRODUCTION:Drosophila Singed (mammalian Fascin) is an actin-binding protein that is known mainly for bundling parallel actin filaments. Among many functions of Singed, it is required for cell motility for both Drosophila and mammalian systems. Increased Fascin-1 levels positively correlate with greater metastasis and poor prognosis in human cancer. Border cell cluster, forms and migrates during Drosophila egg chamber development, shows higher expression of Singed compared with other follicle cells. Interestingly, loss of singed in border cells does not lead to any effect other than delay.
RESULT:In this work, we have screened many actin-binding proteins in search of functional redundancy with Singed for border cell migration. We have found that Vinculin works with Singed to regulate border cell migration, albeit mildly. Although Vinculin is known for anchoring F-actin to the membrane, knockdown of both singed and vinculin leads to a reduced level of F-actin and changes in protrusion characteristics in border cells. We have also observed that they may act together to control microvilli length of brush border membrane vesicles and the shape of egg chambers in Drosophila.
CONCLUSIONS:We may conclude that singed and vinculin work together to control F-actin and these interactions are consistent across multiple platforms.
Published 2023-3-13
DOI 10.1002/dvdy.585
PMID 36912821
IF 3.275
Drosophila DGRC#104 055 DGRC#108 561