RRC ID 74726
Author Ogawa A, Kojima F, Miyake Y, Yoshimura M, Ishijima N, Iyoda S, Sekine Y, Yamanaka Y, Yamamoto K.
Title Regulation of constant cell elongation and Sfm pili synthesis in Escherichia coli via two active forms of FimZ orphan response regulator.
Journal Genes Cells
Abstract Escherichia coli (E. coli) has multiple copies of the chaperone-usher (CU) pili operon in five fimbria groups: CU pili, curli, type IV pili, type III secretion pili, and type IV secretion pili. Commensal E. coli K-12 contains 12 CU pili operons. Among these operons, Sfm is expressed by the sfmACDHF operon. Transcriptome analyses, reporter assays, and chromatin immunoprecipitation PCR analyses reported that FimZ directly binds to and activates the sfmA promoter, transcribing sfmACDHF. In addition, FimZ regularly induces constant cell elongation in E. coli, which is required for F-type ATPase function. The bacterial two-hybrid system showed a specific interaction between FimZ and the α subunit of the cytoplasmic F1 domain of F-type ATPase. Studies performed using mutated FimZs have revealed two active forms, I and II. Active form I is required for constant cell elongation involving amino acid residues K106 and D109. Active form II additionally required D56, a putative phosphorylation site, to activate the sfmA promoter. The chromosomal fimZ was hardly expressed in parent strain but functioned in phoB and phoP double-gene knockout strains. These insights may help to understand bacterial invasion restricted host environments by the sfm γ-type pili.
Volume 27(11)
Pages 657-674
Published 2022-11-1
DOI 10.1111/gtc.12982
PMID 36057789
MeSH Adenosine Triphosphatases / genetics Bacterial Proteins / genetics Bacterial Proteins / metabolism Copper / metabolism Escherichia coli* / genetics Escherichia coli* / metabolism Escherichia coli Proteins* / genetics Escherichia coli Proteins* / metabolism Fimbriae Proteins / genetics Fimbriae Proteins / metabolism Gene Expression Regulation, Bacterial Molecular Chaperones / genetics
IF 1.655
Prokaryotes E. coli pBADFimZ-D56A pBADFimZ-D56E pBADFimZ-D56W pBADFimZ-D56Q pBADFimZ-D56N pBADFimZ-D58A pBADFimZ-D86A pBADFimZ-D88A pBADFimZ-K106A pBADFimZ-D109A pBADFimZ-175 pLux-sfmA pLux-puuA pLux-puuC pLux-puuD pLux-arcA pLux-arcB pLux-atoS pLux-mdtA pLux-barA pLux-uvrY pLux-basR pLux-citA pLux-cpxR pLux-creA pLux-cusR pLux-dcuS pLux-ompR pLux-evgA pLux-fimZ pLux-zraS pLux-kdpD pLux-narQ pLux-narP pLux-narX pLux-glnA pLux-phoP pLux-phoB pLux-qseB pLux-rcsD pLux-rcsB pLux-rcsC pLux-rstA pLux-torS pLux-torR pLux-ivbL pLux-hprR pLux-btsS pLux-glrK pLux-glrR pLux-pyrS pT18 pT25 pT25-fimZ pT25-fimZ-D56A pT25-fimZ-K106A pT25-fimZ-D109A pT18-atpA pT18-aptD psgRNA-atpD psgRNA-atpE psgRNA-fimZ ME9939 ME9940 ME9941 ME9942 ME9943 ME9944 ME9945 ME9946 ME9947 ME9948 ME9949 ME9950 pGRPK049 pBADFimZ pBADFimZ-D10A pBADFimZ-H12A pBADFimZ-K34A pBADFimZ-D36A pBADFimZ-D37A