論文 - 詳細
| RRC ID | 74730 |
|---|---|
| 著者 | Kim AA, Nguyen A, Marchetti M, Du X, Montell DJ, Pruitt BL, O'Brien LE. |
| タイトル | Independently paced Ca2+ oscillations in progenitor and differentiated cells in an ex vivo epithelial organ. |
| ジャーナル | J Cell Sci |
| Abstract |
Cytosolic Ca2+ is a highly dynamic, tightly regulated and broadly conserved cellular signal. Ca2+ dynamics have been studied widely in cellular monocultures, yet organs in vivo comprise heterogeneous populations of stem and differentiated cells. Here, we examine Ca2+ dynamics in the adult Drosophila intestine, a self-renewing epithelial organ in which stem cells continuously produce daughters that differentiate into either enteroendocrine cells or enterocytes. Live imaging of whole organs ex vivo reveals that stem-cell daughters adopt strikingly distinct patterns of Ca2+ oscillations after differentiation: enteroendocrine cells exhibit single-cell Ca2+ oscillations, whereas enterocytes exhibit rhythmic, long-range Ca2+ waves. These multicellular waves do not propagate through immature progenitors (stem cells and enteroblasts), of which the oscillation frequency is approximately half that of enteroendocrine cells. Organ-scale inhibition of gap junctions eliminates Ca2+ oscillations in all cell types - even, intriguingly, in progenitor and enteroendocrine cells that are surrounded only by enterocytes. Our findings establish that cells adopt fate-specific modes of Ca2+ dynamics as they terminally differentiate and reveal that the oscillatory dynamics of different cell types in a single, coherent epithelium are paced independently. |
| 巻・号 | 135(14) |
| 公開日 | 2022-7-15 |
| DOI | 10.1242/jcs.260249 |
| PII | 276030 |
| PMID | 35722729 |
| PMC | PMC9450890 |
| MeSH | Animals Calcium* / metabolism Cell Differentiation / physiology Drosophila / metabolism Drosophila Proteins* / metabolism Enteroendocrine Cells / metabolism |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#112304 |