RRC ID 74730
著者 Kim AA, Nguyen A, Marchetti M, Du X, Montell DJ, Pruitt BL, O'Brien LE.
タイトル Independently paced Ca2+ oscillations in progenitor and differentiated cells in an ex vivo epithelial organ.
ジャーナル J Cell Sci
Abstract Cytosolic Ca2+ is a highly dynamic, tightly regulated and broadly conserved cellular signal. Ca2+ dynamics have been studied widely in cellular monocultures, yet organs in vivo comprise heterogeneous populations of stem and differentiated cells. Here, we examine Ca2+ dynamics in the adult Drosophila intestine, a self-renewing epithelial organ in which stem cells continuously produce daughters that differentiate into either enteroendocrine cells or enterocytes. Live imaging of whole organs ex vivo reveals that stem-cell daughters adopt strikingly distinct patterns of Ca2+ oscillations after differentiation: enteroendocrine cells exhibit single-cell Ca2+ oscillations, whereas enterocytes exhibit rhythmic, long-range Ca2+ waves. These multicellular waves do not propagate through immature progenitors (stem cells and enteroblasts), of which the oscillation frequency is approximately half that of enteroendocrine cells. Organ-scale inhibition of gap junctions eliminates Ca2+ oscillations in all cell types - even, intriguingly, in progenitor and enteroendocrine cells that are surrounded only by enterocytes. Our findings establish that cells adopt fate-specific modes of Ca2+ dynamics as they terminally differentiate and reveal that the oscillatory dynamics of different cell types in a single, coherent epithelium are paced independently.
巻・号 135(14)
公開日 2022-7-15
DOI 10.1242/jcs.260249
PII 276030
PMID 35722729
PMC PMC9450890
MeSH Animals Calcium* / metabolism Cell Differentiation / physiology Drosophila / metabolism Drosophila Proteins* / metabolism Enteroendocrine Cells / metabolism
IF 4.573
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