論文 - 詳細
| RRC ID | 74761 |
|---|---|
| 著者 | Dai Y, Wu S, Cao C, Xue R, Luo X, Wen Z, Xu J. |
| タイトル | Csf1rb regulates definitive hematopoiesis in zebrafish. |
| ジャーナル | Development |
| Abstract |
In vertebrates, hematopoietic stem and progenitor cells (HSPCs) are capable of self-renewal and continuously replenishing all mature blood lineages throughout life. However, the molecular signaling regulating the maintenance and expansion of HSPCs remains incompletely understood. Colony-stimulating factor 1 receptor (CSF1R) is believed to be the primary regulator for the myeloid lineage but not HSPC development. Here, we show a surprising role of Csf1rb, a zebrafish homolog of mammalian CSF1R, in preserving the HSPC pool by maintaining the proliferation of HSPCs. Deficiency of csf1rb leads to a reduction in both HSPCs and their differentiated progenies, including myeloid, lymphoid and erythroid cells at early developmental stages. Likewise, the absence of csf1rb conferred similar defects upon HSPCs and leukocytes in adulthood. Furthermore, adult hematopoietic cells from csf1rb mutants failed to repopulate immunodeficient zebrafish. Interestingly, loss-of-function and gain-of-function assays suggested that the canonical ligands for Csf1r in zebrafish, including Csf1a, Csf1b and Il34, were unlikely to be ligands of Csf1rb. Thus, our data indicate a previously unappreciated role of Csf1r in maintaining HSPCs, independently of known ligands. |
| 巻・号 | 149(16) |
| 公開日 | 2022-8-15 |
| DOI | 10.1242/dev.200534 |
| PII | 276084 |
| PMID | 35876681 |
| MeSH | Animals Cell Differentiation / physiology Hematopoiesis / genetics Hematopoietic Stem Cells / physiology Mammals Zebrafish* / genetics Zebrafish Proteins* / genetics |
| IF | 5.611 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(kdrl:NLS-Eos)ncv6Tg |