RRC ID 74777
Author Serna-Morales E, Sánchez-Sánchez BJ, Marcotti S, Nichols A, Bhargava A, Dragu A, Hirvonen LM, Díaz-de-la-Loza MD, Mink M, Cox S, Rayfield E, Lee RM, Hobson CM, Chew TL, Stramer BM.
Title Extracellular matrix assembly stress initiates Drosophila central nervous system morphogenesis.
Journal Dev Cell
Abstract Forces controlling tissue morphogenesis are attributed to cellular-driven activities, and any role for extracellular matrix (ECM) is assumed to be passive. However, all polymer networks, including ECM, can develop autonomous stresses during their assembly. Here, we examine the morphogenetic function of an ECM before reaching homeostatic equilibrium by analyzing de novo ECM assembly during Drosophila ventral nerve cord (VNC) condensation. Asymmetric VNC shortening and a rapid decrease in surface area correlate with the exponential assembly of collagen IV (Col4) surrounding the tissue. Concomitantly, a transient developmentally induced Col4 gradient leads to coherent long-range flow of ECM, which equilibrates the Col4 network. Finite element analysis and perturbation of Col4 network formation through the generation of dominant Col4 mutations that affect assembly reveal that VNC morphodynamics is partially driven by a sudden increase in ECM-driven surface tension. These data suggest that ECM assembly stress and associated network instabilities can actively participate in tissue morphogenesis.
Published 2023-4-17
DOI 10.1016/j.devcel.2023.03.019
PII S1534-5807(23)00135-1
PMID 37086718
IF 10.092
Drosophila DGRC#102873