| 著者 |
Scaramella C, Alzagatiti JB, Creighton C, Mankatala S, Licea F, Winter GM, Emtage J, Wisnieski JR, Salazar L, Hussain A, Lee FM, Mammootty A, Mammootty N, Aldujaili A, Runnberg KA, Hernandez D, Zimmerman-Thompson T, Makwana R, Rouvere J, Tahmasebi Z, Zavradyan G, Campbell CS, Komaranchath M, Carmona J, Trevitt J, Glanzman D, Roberts AC.
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| Abstract |
Because of their ex utero development, relatively simple nervous system, translucency, and availability of tools to investigate neural function, larval zebrafish are an exceptional model for understanding neurodevelopmental disorders and the consequences of environmental toxins. Furthermore, early in development, zebrafish larvae easily absorb chemicals from water, a significant advantage over methods required to expose developing organisms to chemical agents in utero Bisphenol A (BPA) and BPA analogs are ubiquitous environmental toxins with known molecular consequences. All humans have measurable quantities of BPA in their bodies. Most concerning, the level of BPA exposure is correlated with neurodevelopmental difficulties in people. Given the importance of understanding the health-related effects of this common toxin, we have exploited the experimental advantages of the larval zebrafish model system to investigate the behavioral and anatomic effects of BPA exposure. We discovered that BPA exposure early in development leads to deficits in the processing of sensory information, as indicated by BPA's effects on prepulse inhibition (PPI) and short-term habituation (STH) of the C-start reflex. We observed no changes in locomotion, thigmotaxis, and repetitive behaviors (circling). Despite changes in sensory processing, we detected no regional or whole-brain volume changes. Our results show that early BPA exposure can induce sensory processing deficits, as revealed by alterations in simple behaviors that are mediated by a well-defined neural circuit.
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